2/14/2023 0 Comments Uncharted 4 iggIL-1, 40 ISEM, and IL-1 antibody were used to stimulate HFL-1 cells and the expression of PRMT1 was detected by RT-qPCR and Western blotting (C,D). The expression of PRMT1 in HFL-1 cells was determined by RT-qPCR after 24h stimulation by different concentrations of ISEM (20, 40, 60, 80, 100) (B). Uncharted 4 Igg Download View Publication.Uncharted 4 Igg Download View PublicationDownload View publication Copy reference Copy caption Embed figure Expression of IL-4 receptor and IL-1 receptor I in BEAS-2B and HFL-1 cells and expression of PRMT1 after stimulation with ISEM in HFL-1 cells.The expression of IL-4 receptor and IL-1 receptor I were detected by Western blotting with or without IL-4 and IL-1 stimulation in BEAS-2B and HFL-1cells (A).The supernatant from IL-4-stimulated BEAS-2B cells (ISEM) was accumulated after 48 hours stimulation and 5 FBS was added to the culture supernatant to replenish the consumption by epithelial cells. Methylated arginines are involved in diverse biological functions such as transcriptional regulation, DNA repair, signal transduction and protein-protein interactions 16. We will review new knowledge on protein arginine methylation and its consequential contribution to therapeutic resistance of pancreatic cancer, underlying molecular mechanism, and clinical application of potential strategies of its reversal.
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